| Software | Key Feature | Platform | |----------|-------------|----------| | Primer3Plus | Web-based, simple interface | Online | | BatchPrimer3 | High-throughput design | Linux/Web | | NCBI Primer-BLAST | Specificity check against genomes | Online | | GenScript Real-time PCR Primer Tool | TaqMan support | Free with account |

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: The Primer Express v3.0 Getting Started Guide provides detailed instructions on installation and initial configuration.

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